MOLECULAR ONCOLOGY
E2F/CHR promoter architecture and cell-cycle timing

Research programme 02

E2F/CHR promoter architecture and cell-cycle timing

We investigate how compact promoter elements encode phase-specific repression and activation from G1 through cytokinesis.

Central question

How do E2F, CDE and CHR elements organise the timing and amplitude of human cell-cycle gene expression?

Cell-cycle promoters contain regulatory elements that function as molecular logic gates. E2F sites are central to G1/S control, while CHR elements recruit MuvB-containing complexes and are especially important for late cell-cycle genes.

Experimental dissection of the Ki-67 promoter and its CDE/CHR elements. Figure 3 from Uxa et al., Cell Death & Differentiation (2021), “Ki-67 gene expression.” Reused under CC BY 4.0.
Experimental dissection of the Ki-67 promoter and its CDE/CHR elements. Figure 3 from Uxa et al., Cell Death & Differentiation (2021), “Ki-67 gene expression.” Reused under CC BY 4.0.

Approaches

Promoter-reporter assaysSite-directed mutagenesisDNA-affinity purification

Selected work

The p53-p21-DREAM-CDE/CHR pathway regulates G2/M cell cycle genes (2016)
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